PM 7/90 (2) Anisogramma anomala.

This document is a diagnostic protocol for Anisogramma anomala, a pathogen that causes eastern filbert blight on European hazelnut trees. It provides information on identifying and detecting the pathogen, including its symptoms and characteristics. The document includes molecular tests for confirmation in critical cases and emphasizes the importance of early detection. It also provides guidance on reporting and documentation, as well as contact information for further information. The appendices provide instructions for isolating the organism and describe the media used for its growth. The document also includes instructions for conducting a real-time PCR test to detect A. anomala, with information on controls, interpretation of results, and performance characteristics. The test has been validated and shown to have high sensitivity and specificity. [Extracted from the article]