The Impact of Diabetes on Male Silkworm Reproductive Health.

Simple Summary: Diabetic male reproductive damage is a prevalent complication of type II diabetes, prompting a multitude of experimental investigations. However, the use of mammalian models for studying diabetic reproductive damage is expensive and raises ethical concerns. Hence, the identification of alternative animal models is imperative. The scientific community is increasingly recognizing the use of silkworms Bombyx mori as animal models, primarily because of their numerous advantages, including low cost and fewer ethical concerns. This study established a type II diabetic silkworm model via a high-glucose diet. The impact of diabetes on the reproductive system of male silkworms was observed, confirming the potential for using silkworms to create a model for diabetic reproductive damage. The increasing prevalence of diabetic reproductive complications has prompted the development of innovative animal models. The use of the silkworm Bombyx mori as a model for diabetic reproductive damage shows potential as a valuable research tool. This study employed silkworms as a novel model to investigate diabetic reproductive damage. The silkworms were fed a high-glucose diet containing 10% glucose to induce a diabetic model. Subsequently, the study concentrated on assessing the influence of diabetes on the reproductive system of male silkworms. The results indicate that diabetes resulted in reduced luteinizing hormone (LH) and testosterone (T) levels, as well as elevated triglyceride (TG) levels in male silkworms. Moreover, diabetes mellitus was associated with pathological testicular damage in male silkworms, accompanied by decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels, along with increased malondialdehyde (MDA) levels in the testis. Additionally, diabetes mellitus reduced the expression of siwi1 and siwi2 genes in the testis of male silkworms. Overall, these results support using silkworms as a valuable model for studying diabetic reproductive damage. [ABSTRACT FROM AUTHOR]