Isolation of sulfate polysaccharides (Ulvan) from the green algae of Ulva lactuca and characterization of its functional groups.

Ulvan is a water-soluble polysaccharide containing a sulfate group isolated from Ulva, a green alga with unique structural properties and the potential to be developed in biomaterials supported by biological activities such as antioxidant, antibacterial, anticoagulant and immunomodulatory. This research aims to isolate ulvan from Ulva lactuca and to study the characteristic of the ulvan functional groups. The methods used were extraction with hydrochloride acid 0.1 M and precipitation using ethanol 96% to obtain a percent yield of ulvan. The ulvan structure was characterized by UV-Vis and FTIR spectrophotometry. The amount of sugar was determined using phenol-sulfuric acid method. The results showed the yield of ulvan produced about 23.34% at maximum UV-Vis wavelength of 205 nm and the FTIR spectra significant at 854.47-1097.50 cm−1 of sulphated polysaccharides region. The sugar content of the isolated ulvan which extracted using hydrochloric acid 0.1 M was 1.4124 mg/mL. [ABSTRACT FROM AUTHOR]