自动顶空-气相色谱法测定人尿中 1，1，2，2-四氯乙烷.

Objective To establish an automated headspace -gas chromatography method for the determination of 1,1,2,2 - tetrachloroethene in human urine. Methods A urine sample of 5.00 mL was placed in a 20 mL headspace vial, and 2.0 g of anhydrous sodium sulfite was added before sealing the vial. The vial was placed in an automated headspace sampler and heated at 80 ℃ for 50.00 min to equilibrate. Under these heating conditions, 1,1,2,2 -tetrachloroethene in human urine was completely reacted with anhydrous sodium sulfite to form trichloroethylene. The trichloroethylene vapor above the headspace vial was separated by a DB-624 capillary column and detected with a flame ionization detector. Quantification was achieved by plotting a standard curve with the peak area of trichloroethylene and the mass concentration of 1,1,2,2- tetrachloroethane. Results The results showed good linear ranges of 1,1,2,2 -tetrachloroethene in urine between 0.008 3 and 32.000 mg/L, and the correlation coefficient was higher than 0.999 4. For this method, the limit of detection was 2.5 μg/L, and the lower limit of quantification was 8.3 μg/L; the intra-batch precision was 4.79% to 5.28%, and the interbatch precision was 4.61% to 6.44% ; the average recovery rate was 87.53% to 101.17% . Conclusions The automated headspace gas chromatography method for determining 1,1,2,2-tetrachloroethene in human urine was characterized by high sensitivity, good linearity, minimal interference, high precision, and simple sample preparation. It is suitable for measuring the concentration of 1,1,2,2-tetrachloroethene in human urine. [ABSTRACT FROM AUTHOR]