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Molecular features, antiviral activity, and immunological expression assessment of interferon-related developmental regulator 1 (IFRD1) in red-spotted grouper (Epinephelus akaara).

Authors :
Hanchapola, H.A.C.R.
Kim, Gaeun
Liyanage, D.S.
Omeka, W.K.M.
Udayantha, H.M.V.
Kodagoda, Y.K.
Dilshan, M.A.H.
Rodrigo, D.C.G.
Jayamali, B.P.M. Vileka
Kim, Joungeun
Jeong, Taehyug
Lee, Sukkyoung
Qiang, Wan
Lee, Jehee
Source :
Fish & Shellfish Immunology. Oct2024, Vol. 153, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

Interferon-related developmental regulator 1 (IFRD1) is a viral responsive gene associated with interferon-gamma. Herein, we identified the IFRD1 gene (EaIFRD1) from red-spotted grouper (Epinephelus akaara), evaluated its transcriptional responses, and investigated its functional features using various biological assays. EaIFRD1 encodes a protein comprising 428 amino acids with a molecular mass of 48.22 kDa. It features a substantial domain belonging to the interferon-related developmental regulator superfamily. Spatial mRNA expression of EaIFRD1 demonstrated the highest expression levels in the brain and the lowest in the skin. Furthermore, EaIFRD1 mRNA expression in grouper tissues exhibited significant modulation in response to immune stimulants, including poly (I:C), LPS, and nervous necrosis virus (NNV) infection. We analyzed downstream gene regulation by examining type Ⅰ interferon pathway genes following EaIFRD1 overexpression. The results demonstrated a significant upregulation in cells overexpressing EaIFRD1 compared to the control after infection with viral hemorrhagic septicemia virus (VHSV). A subcellular localization assay confirmed the nuclear location of the EaIFRD1 protein, consistent with its role as a transcriptional coactivator. Cells overexpressing EaIFRD1 exhibited increased migratory activity, enhancing wound-healing capabilities compared to the control. Additionally, under H 2 O 2 exposure, EaIFRD1 overexpression protected cells against oxidative stress. Overexpression of EaIFRD1 also reduced poly (I:C)-mediated NO production in RAW267.4 macrophage cells. In FHM cells, EaIFRD1 overexpression significantly reduced VHSV virion replication. Collectively, these findings suggest that EaIFRD1 plays a crucial role in the antiviral immune response and immunological regulation in E. akaara. • Interferon-related developmental regulator 1 (IFRD1) in the red spotted grouper was identified and characterized. • EaIFRD1 mRNA expression upregulated significantly in peripheral blood, liver, and spleen cells after the immune challenge. • EaIFRD1 upregulates the wound-healing activity in FHM cells. • EaIFRD1 increased the cell viability under oxidative stress in FHM cells and reduced NO production in macrophages. • Overexpression of EaIFRD1 activates ISGs and reduces VHSV replication. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10504648
Volume :
153
Database :
Academic Search Index
Journal :
Fish & Shellfish Immunology
Publication Type :
Academic Journal
Accession number :
179558871
Full Text :
https://doi.org/10.1016/j.fsi.2024.109859