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PB1-F2 of low pathogenicity H7N7 restricts apoptosis in avian cells.
- Source :
-
Virus Research . Nov2024, Vol. 349, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- • In chicken full-length PB1-F2 of LPAIV H7N7 prolonged survival of infected cells by limiting apoptotic cell death. • PB1-F2 knockout LPAIV significantly decreased MHC-I expression on fibroblasts, delayed tissue healing and increased phagocytic uptake of infected cells. • Taken together, the full-length PB1-F2 allows AIV to cause prolonged infections without serious harm to the avian host. Avian influenza viruses (AIV) pose a continuous challenge to global health and economy. While countermeasures exist to control outbreaks in poultry, the persistent circulation of AIV in wild aquatic and shorebirds presents a significant challenge to effective disease prevention efforts. PB1-F2 is a non-structural protein expressed from a second open reading frame (+1) of the polymerase basic 1 (PB1) segment. The sequence and length of the PB1-F2 protein can vary depending on the host of origin. While avian isolates typically carry full-length PB1-F2, isolates from mammals, often express truncated forms. The selective advantage of the full-length PB1-F2 in avian isolates is not fully understood. Most research on the role of PB1-F2 in influenza virus replication has been conducted in mammalian systems, where PB1-F2 interfered with the host immune response and induced apoptosis. Here, we used Low Pathogenicity (LP) AIV H7N7 expressing full-length PB1-F2 as well as a knockout mutant. We found that the full-length PB1-F2 of LPAIV prolonged survival of infected cells by limiting apoptotic cell death. Furthermore, PB1-F2 knockout LPAIV significantly decreased MHC-I expression on fibroblasts, delayed tissue healing and increased phagocytic uptake of infected cells, whereas LPAIV expressing PB1-F2 has limited effects. These findings indicate that full-length PB1-F2 enables AIV to cause prolonged infections without severely harming the avian host. Our observations may explain maintenance of AIV in the natural bird reservoir in absence of severe clinical signs. [Display omitted] [ABSTRACT FROM AUTHOR]
- Subjects :
- *AVIAN influenza A virus
*CELL death
*CHICKENS
*FIBROBLASTS
*VIRAL replication
Subjects
Details
- Language :
- English
- ISSN :
- 01681702
- Volume :
- 349
- Database :
- Academic Search Index
- Journal :
- Virus Research
- Publication Type :
- Academic Journal
- Accession number :
- 179734027
- Full Text :
- https://doi.org/10.1016/j.virusres.2024.199444