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Time to resolution of airway inflammation caused by bronchoalveolar lavage in healthy horses.

Authors :
Woodrow, Jane S.
Hopster, Klaus
Palmisano, Megan
Payette, Flavie
Kulp, Jeaneen
Stefanovski, Darko
Nolen‐Walston, Rose
Source :
Journal of Veterinary Internal Medicine. Sep/Oct2024, Vol. 38 Issue 5, p2776-2782. 7p.
Publication Year :
2024

Abstract

Background: Bronchoalveolar lavage (BAL) is a common procedure for evaluation of the equine lower airways. Time to resolution of post‐BAL inflammation has not been clearly defined. Hypothesis: Residual inflammation, evident by changes in immune cell populations and inflammatory cytokines, will resolve by 72 hours after BAL. Animals: Six adult, healthy, institution‐owned horses. Methods: Randomized, complete cross‐over design. Each horse underwent 3 paired BALs, including a baseline and then 48, 72, and 96 hours later, with a 7‐day washout between paired BALs. Each sample underwent cytological evaluation and cytokine concentrations were determined by a commercially available multiplex bead immunoassay. Statistical analysis was performed by multilevel mixed‐effects Poisson regression analysis. Data are reported as marginal means and 95% confidence interval (CI). Results: Neutrophil, eosinophil and mast cell percentages were not significantly different at any time points. Macrophage percentages were higher at 72 hours (45.0 [95% CI, 41.6‐48.4]%) and 96 hours (45.3 [95% CI, 42.9‐47.7]%) vs baseline (37.4 [95% CI, 33.5‐41.4]%; P <.001 and P =.01, respectively), and at 72 hours and 96 hours vs 48 hours (31.9 [95% CI, 28.1‐35.6]%; P <.001). Neutrophil percentage was not significantly increased at 48 hours (P =.11). Interleukin (IL)‐6 concentration was increased at 72 hours (5.22 [95% CI, 3.44‐6.99] pg/mL) vs 48 hours (4.38 [95% CI, 2.99‐5.78] pg/mL; P <.001). Conclusions and Clinical Importance: Significant lung inflammation was not detected at 72 and 96 hours, suggesting that repeating BAL at 72 hours or more can be done without concern of residual inflammation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
08916640
Volume :
38
Issue :
5
Database :
Academic Search Index
Journal :
Journal of Veterinary Internal Medicine
Publication Type :
Academic Journal
Accession number :
179878032
Full Text :
https://doi.org/10.1111/jvim.17169