Experimental study on the mechanical behavior of EICP-casein-treated calcareous sand.

In order to protect and develop marine resources, it is essential to reinforce the calcareous sand foundation on coral reefs during engineering construction. This study proposes a novel approach that combines enzyme-induced carbonate precipitation (EICP) with casein to solidify calcareous sand, which overcomes the issues of low calcium carbonate crystallization and insufficient cementation strength encountered in the pure EICP method. This study investigated the reinforcement effects and mechanisms of the combined method across various scales. Sonic time testing revealed an initial decline in sample density followed by a gradual increase, with the lowest density observed at 10% casein content. Sample strength depends on casein dosage and curing time. Overall, the trend in sample strength showed an initial decrease followed by a gradual increase, with 10% casein content again resulting in the lowest strength. The measured experimental groups achieved a minimum strength of approximately 1.17 MPa and a maximum strength of around 6.34 MPa. As casein concentration rises, the optimal curing time increases from 12–48 h. Microscopic experiments reveal casein expansion and dissolution in the EICP liquid, explaining UCS value changes with casein dosage and curing time. Combining scanning electron microscopy and microscopic experiments highlights the cementitious material's vital role in strengthening samples. In conclusion, combining EICP and casein for solidifying calcareous sand is technically feasible and highly effective with significant reinforcement effects. [ABSTRACT FROM AUTHOR]