Overexpression of Aterf022 Gene Regulates Fruit Ripening and Nutritional Quality in Tomato Solanum lycopersicum.

Tomato Solanum lycopersicum is an important economic fruit, and widely consumed for its high nutritional quality. Our previous study found that AtERF022, a member of Arabidopsisthaliana ethylene response factors (ERFs) group III, acted a vital role in Arabidopsis growth and development. However, whether or how endogenous AtERF022 confers tomato fruit quality is still elusive. To explore the role of AtERF022 gene in tomato fruit ripening and nutritional quality, we generated AtERF022-overexpressing tomato line by transgenic and plant tissue culture technology. Our results showed that the higher expression of AtERF022 was found in tomato flowers and fruits, especially in orange ripening stage of fruits. Compared to wild-type, AtERF022-overexpressing tomato exhibited morphological growth advantages and elevated fruit ripening and nutritional quality. Further experiments and transcriptome sequencing showed that overexpression of AtERF022 enhanced the expression of genes involved in ethylene biosynthesis, cell wall metabolism, chlorophyll degradation, carotenoids and flavonoids biosynthesis, and thereby promoting fruit ripening, β-carotene, flavonoids, vitamin C and total phenols accumulation. Together, our results provide genetic evidence that AtERF022 regulates tomato fruit ripening and nutritional quality. [ABSTRACT FROM AUTHOR]