Indirect immunofluorescence for the diagnosis of bullous pemphigoid and mucous membrane pemphigoid patients: human or monkey salt‐split skin sections.

The article explores the use of indirect immunofluorescence on salt-split skin sections to diagnose bullous pemphigoid (BP) and mucous membrane pemphigoid (MMP) in patients. The study compares the sensitivity and specificity of human and monkey skin samples in detecting autoantibodies from BP and MMP patients. While human skin proved more sensitive in detecting BP autoantibodies, monkey skin showed higher sensitivity for MMP autoantibodies due to differences in antigen expression. The study suggests carefully selecting the appropriate substrate to enhance the diagnostic performance of the immunofluorescence technique. [Extracted from the article]