Exercise Mitigates Apoptosis Induced by Lipid Peroxidation in Ameloblasts of Fluorosis Mice.

Purpose: Excessive fluoride ingestion induces dental fluorosis that is generated by oxidative stress-mediated apoptosis. Oppositely, regular exercise has been shown to ameliorate oxidative damages and alleviate cell apoptosis in various diseases. However, little attention is paid to the effect of exercise on dental fluorosis. Thus, we employed the exercise regime including forced treadmill running (FTR) in fluorosis mice to explore the effect. Methods: Briefly, three-week-old Institute of Cancer Research (ICR) mice drinking water contained 100 mg/L sodium fluoride (NaF) and 0 mg/L NaF were compulsively submitted to treadmill running for 12 months. Results: Results showed that 12 months of fluoride exposure markedly elevated the incisors fluorine concentrations. FTR restrained the elevation of cross-linked C-terminal telopetide of type I collagen (CTX-I) concentration in urine from mice after 12 months of fluoride exposure. The slightly reduced contents of reactive oxygen species (ROS), malondialdehyde (MDA) and observably enhanced activities of catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and total antioxidant capacity (T-AOC) were found in the incisors of fluoride-toxic mice subjected to FTR. Furthermore, FTR restrained fluoride-caused ameloblasts apoptosis induced by MDA in incisors through reducing the mRNA expressions of p38 mitogen-activated protein kinase (p38MAPK), c-Jun N-terminal kinase (JNK), IkappaB kinase-beta (IKK-β), Caspase3 and enhancing the mRNA expression of B-cell lymphoma 2 (Bcl-2), superoxide dismutase 1 (SOD1). Conclusions: Above results suggested that regular exercise may be a convenient therapy for dental fluorosis. [ABSTRACT FROM AUTHOR]