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GhSBI1, a CUP‐SHAPED COTYLEDON 2 homologue, modulates branch internode elongation in cotton.

Authors :
Zhong, Weiping
Wu, Lanxin
Li, Yan
Li, Xiaxuan
Wang, Junyi
Pan, Jingwen
Zhu, Shouhong
Fang, Shentao
Yao, Jinbo
Zhang, Yongshan
Chen, Wei
Source :
Plant Biotechnology Journal. Nov2024, Vol. 22 Issue 11, p3175-3193. 19p.
Publication Year :
2024

Abstract

Summary: Branch length is an important plant architecture trait in cotton (Gossypium) breeding. Development of cultivars with short branch has been proposed as a main object to enhance cotton yield potential, because they are suitable for high planting density. Here, we report the molecular cloning and characterization of a semi‐dominant quantitative trait locus, Short Branch Internode 1(GhSBI1), which encodes a NAC transcription factor homologous to CUP‐SHAPED COTYLEDON 2 (CUC2) and is regulated by microRNA ghr‐miR164. We demonstrate that a point mutation found in sbi1 mutants perturbs ghr‐miR164‐directed regulation of GhSBI1, resulting in an increased expression level of GhSBI1. The sbi1 mutant was sensitive to exogenous gibberellic acid (GA) treatments. Overexpression of GhSBI1 inhibited branch internode elongation and led to the decreased levels of bioactive GAs. In addition, gene knockout analysis showed that GhSBI1 is required for the maintenance of the boundaries of multiple tissues in cotton. Transcriptome analysis revealed that overexpression of GhSBI1 affects the expression of plant hormone signalling‐, axillary meristems initiation‐, and abiotic stress response‐related genes. GhSBI1 interacted with GAIs, the DELLA repressors of GA signalling. GhSBI1 represses expression of GA signalling‐ and cell elongation‐related genes by directly targeting their promoters. Our work thus provides new insights into the molecular mechanisms for branch length and paves the way for the development of elite cultivars with suitable plant architecture in cotton. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14677644
Volume :
22
Issue :
11
Database :
Academic Search Index
Journal :
Plant Biotechnology Journal
Publication Type :
Academic Journal
Accession number :
180410810
Full Text :
https://doi.org/10.1111/pbi.14439