An absolute quantification method for selected MFGM proteins in infant formula using targeted proteomics.

Milk fat globule membrane (MFGM) proteins are receiving increased attention due to their reported benefits for human health, particularly in infant populations. Challenges exist in MFGM protein quantification due to their low quantities, complex chemistry, interactions with other matrix components, and the high matrix complexity. In this study, a subset of four MFGM proteins were selected as relevant targets for identification and quantification in an infant formula (IF) matrix: butyrophilin, mucin 1, xanthine dehydrogenase/oxidase, and perilipin 2. An analytical protocol for absolute quantification of these four MFGM proteins in IF was successfully developed and validated. Additionally, the feasibility to apply the method in selected MFGM ingredients was also demonstrated. The method demonstrated good performance; high accuracy and robustness, low LOQ and uncertainty, while controlling the matrix effect. This study represents a report of an analytical method to quantify selected MFGM proteins in IF. • The matrix effect from infant formula had a major impact on protein quantification. • The method was also applied for use in milk fat globule membrane ingredients. • Of the four proteins studied, Butyrophilin had the highest concentration. • Of the four proteins studied, Mucin 1 had the lowest concentration. • The method showed high sensitivity and precision, and low uncertainty. [ABSTRACT FROM AUTHOR]