Synthesis and Binding Properties of High‐Affinity Histidine‐Bearing Polymers for Wood Lignin.

The pursuit of molecules capable of binding to wood lignin is pivotal for advancing lignin degradation technology, particularly when combined with lignin degradation catalysts. In this study, synthetic polymers bearing histidine moieties, demonstrating remarkable affinity for wood lignin are reported. These polymers, featuring varying degrees of histidine substitution in the form of histidine methyl esters, are synthesized through controlled radical polymerization of an activated ester‐bearing monomer, employing a fluorescein‐labeled chain transfer agent and subsequent postpolymerization amidation with histidine methyl ester. The binding properties of these histidine‐bearing polymers with milled wood lignin under aqueous conditions are investigated. Qualitative assessment of lignin‐binding capabilities involve spectroscopic analysis of changes in absorbance of visible light and fluorescence intensity. Furthermore, quantitative evaluation is conducted through surface plasmon resonance measurements to determine the binding parameters of the polymers with wood lignin. Notably, polymers with higher histidine substitution exhibit enhanced binding affinity compared to those with lower histidine substitution levels. [ABSTRACT FROM AUTHOR]