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The homeostasis of ions and reactive oxygen species in root and shoot play crucial roles in the tolerance of alfalfa to salt alkali stress.

Authors :
Zhe, Zhang
Hongjiao, Zhang
Tongtong, Yao
Kexin, Wang
Jingjing, Xu
Hongrui, Zhang
Siyue, Qi
Hong, Ao
Bo, Qin
Huihui, Zhang
Source :
Plant Physiology & Biochemistry. Nov2024, Vol. 216, pN.PAG-N.PAG. 1p.
Publication Year :
2024

Abstract

High pH saline-alkali stress, mainly NaHCO 3 , limited the development of animal husbandry in Songnen Plain. Ion imbalance and reactive oxygen species (ROS) metabolism disorder caused by saline-alkali stress inhibited plant growth. In this study, we compared the differences in ion absorption, transport and ROS metabolism between saline-tolerant alfalfa (ZD) and saline-sensitive alfalfa (ZM) under NaHCO 3 stress using physiology and transcripomics techniques. WGCNA analysis identified key genes associated with NaHCO 3 stress-induced changes. NaHCO 3 stress inhibited the absorption of K+ and Mg2+, but activated Ca2+ signal. Furthermore, ZD maintained higher K+, Mg2+ and Ca2+ contents and the K+/Na+ ratio than ZM, this is mainly related to the higher expression of proteins or channel-encoding genes involved in ion absorption and transport in ZD. Antioxidant enzyme systems can be activated in response to NaHCO 3 stress. Peroxidase (EC 1.11.1.6), catalase (EC 1.11.1.7) and glutathione transferase (EC 2.5.1.18) activities were higher in ZD than ZM, and most genes encoding the relevant enzymes also demonstrated a stronger up-regulation trend in ZD. Although NaHCO 3 stress inhibited Trx-Prx pathway, ZD related enzymes and their genes were also inhibited less than ZM. WGCNA results identified many genes involved in ion absorption, transport and antioxidant systems that play an important role in NaHCO 3 stress adaptation. Collectively, ZD has the stronger ion homeostasis regulation and ROS scavenging ability, so it's more resistant to NaHCO 3. The results provide theoretical guidance for further understanding of the molecular mechanism of NaHCO 3 resistance and provide potential genes for research to improve saline-alkali tolerance in alfalfa. [Display omitted] • NaHCO 3 stress inhibited the absorption of K+ and Mg2+, resulting in a lower K+/Na+ ratio. • Salt-tolerant alfalfa had higher Mg2+, Ca2+ contents and the K+/Na+ ratio than saline-sensitive alfalfa under NaHCO 3 stress. • NaHCO 3 stress reduced ROS accumulation through AsA-GSH cycle but inhibited the Trx-Prx pathway in alfalfa. • Under NaHCO 3 stress, ZD maintained higher antioxidant enzyme activities and related coding genes expression levels in than ZM. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09819428
Volume :
216
Database :
Academic Search Index
Journal :
Plant Physiology & Biochemistry
Publication Type :
Academic Journal
Accession number :
180630755
Full Text :
https://doi.org/10.1016/j.plaphy.2024.109175