The decrease in peripheral blood basophils in a mouse model of IgE-induced inflammation involves their migration to lymph nodes.

During the active phase of urticaria, a decrease in peripheral blood basophils, known as basopenia, is observed. We previously reported that basopenia occurs as a result of basophils migrating to the skin in a contact dermatitis model where a Th2 response is induced with oxazolone. Although there is currently no established model for urticaria, given that urticaria is an IgE-mediated immediate-type allergic reaction, we aimed to determine whether an IgE-mediated model could reproduce the decrease in basophils in peripheral blood observed during the active phase of urticaria. Mice were pretreated with 2,4,6-trinitrophenylhaptene (TNP)-specific IgE and basophil dynamics were examined following stimulation with TNP-ovalbumin. Mast cell-deficient WBB6F1- Kit W /Kit W-v mice were used to investigate the role of mast cells in this IgE-mediated model. Following stimulation, we observed immediate ear swelling and basopenia after 0.5 hours. However, the number of basophils observed in the skin lesions was low, while a higher number of basophils were observed in the antigen-draining lymph nodes (LN). In mast cell-deficient mice, no increase in basophils in the LN was observed, reflecting reduced antigen influx into the LN, but basophils remained in the skin. In the IgE-mediated mouse model, basopenia was observed, which coincided with the induction of inflammation in the skin. The migration of basophils to the LN in this model suggests that the systemic immune system, including the LN, should be considered when exploring the pathogenesis of urticaria in humans. • Peripheral basopenia during active urticaria was verified using a mouse model. • IgE-mediated stimulation induces immediate skin swelling and peripheral basopenia. • IgE-sensitized basophils migrate to the antigen-draining lymph nodes. • Peripheral basophils decrease upon stimulation even in the absence of mast cells. • Without mast cells, antigen and basophil entry into lymph nodes are both suppressed. [ABSTRACT FROM AUTHOR]