Spectrofluorimetric determination of acetone in water samples with solid-phase extraction and a new benzaldehyde derivative.

A new method is described for acetone (C(CH 3) 2 O) determination in water samples. The method is based on the reaction with 4-(dimethylamino)benzaldehyde (DMAB) in dimethyl sulfoxide (DMSO) in slightly basic medium, resulting in a highly fluorescent compound with fluorescent wavelengths undisturbed by other common fluorescent compounds. Experimental conditions were optimized (reagents concentrations, reaction time) to reach optimal sensitivity. For the analysis of aqueous samples, a preconcentration step of acetone by solid-phase extraction (SPE) followed by an elution step in DMSO was optimized using Isolute ENV + columns. A highly satisfactory low detection limit of 0.014 μM (0.8 μg L−1) was achieved by combining these two steps, with a linear range from 0.048 to 5 μM and relative standard deviations between 5.7 % and 6.9 %. The protocol was validated on complex real water samples such as river water and wastewater, and our fluorimetric method with DMAB was in good agreement with the reference LC-UV method with DNPH. [Display omitted] • New benzaldehyde reagent for sub-micromolar fluorescent detection of acetone. • SPE for acetone preconcentration in aqueous samples described for the first time. • Procedure validated on complex real water samples (river water and wastewater). [ABSTRACT FROM AUTHOR]