The OsMYC2–JA feedback loop regulates diurnal flower‐opening time via cell wall loosening in rice.

SUMMARY: Diurnal flower‐opening time (DFOT), the time of spikelet opening during the day, is an important trait for hybrid rice (Oryza sativa L.) seed production. Hybrids between indica and japonica rice varieties have strong heterosis, but the parental lines usually have different, nonoverlapping DFOTs. This reduces the success of hybrid seed production in crosses between indica and japonica subspecies, thus hindering the utilization of indica and japonica inter‐subspecies heterosis. However, little is known about the molecular mechanisms regulating DFOT in rice. Here, we obtained japonica rice lines with a DFOT 1.5 h earlier than the wild type by overexpressing OsMYC2, a gene encoding a key transcription factor in the jasmonate (JA) signaling pathway. OsMYC2 is activated by JA signaling and directly regulates the transcription of genes related to JA biosynthesis and cell wall metabolism. Overexpressing OsMYC2 led to significantly increased JA contents and decreased cellulose and hemicellulose contents in lodicule cells, as well as the softening of lodicule cell walls. This may facilitate the swelling of lodicules, resulting in early diurnal flower‐opening. These results suggest that the OsMYC2–JA feedback loop regulates DFOT in rice via cell wall remodeling. These findings shed light on the understanding of regulatory mechanism of the DFOT of plants, which should promote the development of indica and japonica varieties suitable for hybrid rice breeding. Significance Statement: OsMYC2 regulates diurnal flower‐opening time by directly regulating the transcription of JA biosynthesis genes and cell wall remodeling genes. This study not only elucidates the mechanism of JA regulating diurnal flower‐opening time but also helps reduce the cost of hybrid seed production and promote the development of indica and japonica rice hybrid breeding. [ABSTRACT FROM AUTHOR]