Proximity Effects and Aggregation of Hamilton‐Receptor Barbiturate Host–Guest Complexes Probed by Cross‐Metathesis and ESI MS Analysis.

The molecular environment around supramolecular bonding systems significantly affects their stability and the assembly of host–guest complexes, most prominent for hydrogen bonds (H‐bonds). Hamilton receptor‐barbiturate host–guest complexes are well‐known in solution, typically forming a 1 : 1 molar ratio complex. However, within a polymer matrix, these complexes can form higher‐order assemblies, deviating from the standard 1 : 1 complex, which are challenging to characterize and often require lab‐intensive methods. In this study, a novel Hamilton receptor (<bold>H</bold>) was equipped with cyclopentene moieties and used as a host to form host–guest complexes (<bold>H−B</bold>) with allobarbital (<bold>B</bold>), followed by covalent crosslinking. UV‐Vis spectroscopy titration experiments in different solvents and at various temperatures revealed that polar solvents containing additional H‐bonding sites significantly reduce the formation of the 1 : 1 <bold>H−B</bold> complex, as indicated by a reduced association constant. Higher‐order aggregates (<bold>HH</bold>‐dimer, <bold>HHH</bold>‐trimer) were subsequently detected via an alkene cross‐metathesis (<bold>CM</bold>) reaction to fix the assemblies covalently, followed by analysis via electrospray ionization mass spectrometry (ESI MS). This two‐step method, firstly via <bold>CM</bold> fixation followed by ESI MS, was extended to study the <bold>H−B</bold> model complex within a polyisobutylene (<bold>PIB</bold>) matrix, presenting a direct method to analyze the complex host–guest assembly in solvent‐free (polymer) environments. [ABSTRACT FROM AUTHOR]