The collaboration and competition between indigenous microorganisms and exogenous anaerobic digester sludge in anaerobic treatment of pickled mustard wastewater at different salinities.

The highly concentrated pickled mustard wastewater presents significant potential for energy recovery, but the stress effect of high osmotic pressure on cell integrity and activity seriously impedes the methane production by anaerobic microorganisms. The survival ability of indigenous microorganisms (IM) in pickled mustard wastewater supports the establishment of anaerobic treatment. Moreover, inoculation of anaerobic digester sludge is a common start-up strategy. However, the effects of exogenous anaerobic sludge on IM are unclear, especially in hypersaline environment. This research aimed to investigate the influence of exogenous anaerobic sludge on the construction, performance, and microbiota at 3% and 5% salinity. And the research focused on the collaboration and competition between exogenous anaerobic sludge and IM. The neutral community model (which explains the formation and evolution of biological communities) indicated that the interaction between exogenous digester sludge microorganisms and IM dominated community assembly. At 3%, the digester sludge collaborated with IM to increase daily COD reduction and biogas production compared with IM group. However, at 5%, the competitive relationship reduced daily COD reduction and biogas production compared with IM group. This study provides a new perspective for the selection of inoculation strategies for exogenous anaerobic digester sludge under different salinity, in order to realize energy conversion from salinity organic wastewater. [Display omitted] • The effect of inoculation strategy was evaluated at 3% and 5% salinity. • The inoculation strategy was analyzed from the perspective of microbial interaction. • The microbial interaction dominated community assembly. • At 3%, digester sludge cooperated with native microbiota to enhance performance. • At 5%, the exogenous inoculation reduced the robustness of the microbial network. [ABSTRACT FROM AUTHOR]