Potato Protein Hydrolysate (PPH902) treatment in adipocytes inhibits lipogenesis by activating AMPK.

• Protein hydrolyzates and biopeptides can provide a source of protein with good functional properties. • PPH902 treatment significantly RTR and activate p-AMPK and inhibit adipogenesis in adipocytes. • PPH902 is an effective anti-lipogenesis and lipolysis-promoting hydrolyzate, which has the potential to develop anti-obesity products. Obesity is a chronic metabolic disease caused by unbalanced caloric intake, which has serious harm to health and increases the risk of hypertension, hyperlipidemia, diabetes and cardiovascular diseases. Previous studies have shown that Alcalase treatment-derived potato protein hydrolysate (PPH) has the effects of regulating blood pressure and blood sugar in animal experiments, and has cardioprotective, hepato-protective activity and inhibited muscle protein degradation. This study aims to screen the optimal conditions isolating potato protein hydrolysate with potential anti-obesity activity by regulating the mechanism of lipogenesis and lipolysis. 9% potato protein (PP) hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments. PPH902 at 1600 ppm does not affect the cell viability of 3T3-L1 adipocytes. PPH902 regulates the expression of lipid production-related transcription factors PPARγ, SREBP-1c, and FAS by activating AMPK, thereby inhibiting lipogenesis, and activating phosphorylated HSL to promote lipolysis to increase lipid metabolism. These results show that PPH902 is an effective anti-lipogenesis and lipolysis-promoting hydrolyzate, which has the potential to develop anti-obesity products. [ABSTRACT FROM AUTHOR]