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母猪饲粮添加益生菌和合生元对子代巴马香猪肌肉氨基酸组成及生长发育相关基因表达的影响.

Authors :
祝倩
李宸健
程雅婷
李锐煊
刘雅婷
孔祥峰
Source :
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao. 2024, Vol. 47 Issue 6, p1149-1158. 10p.
Publication Year :
2024

Abstract

[Objectives] This study aimed to determine the effects of maternal addition with probiotics and synbiotics on the muscular amino acid composition and gene expression of offspring Bama mini-pigs. [Methods] A total of 64 pregnant Bama mini-pigs were selected and randomly divided into control (antibiotic-free basal diet), antibiotic (50 g·t-1 virginiamycin), probiotics (200 mL·d-1 probiotic mixture), and synbiotics (500 g xylo-oligosaccharide per ton diet+200 mL·d-1 probiotics mixture) groups, with 16 sows in each group. The sows were raised individually and fed with the corresponding experimental diets during pregnancy and lactation periods. After weaning, two piglets per litter and thirty-two piglets per group were selected and fed a basal diet (eight pens per group, four pigs per pen) . At 65, 95, and 125 days of age, eight pigs per group were selected and biceps femoris (BF) and psoas major (PM) muscles were sampled to determine hydrolyzed amino acid composition and gene expression. [Results] Compared with the control group, the contents of crude protein, Gly, Ser, Ala, Asp, Glu, Pro, and Ile in the BF muscle and Gly, Glu, Ser, and His in the PM muscle increased in the synbiotics group (P<0.05); the expression levels of muscle atrophy F-box protein 32 and myogenin (MyoG) in the BF muscle and myogenic factor 5 (Myf5) in the PM muscle were upregulated in the probiotics group (P<0.05), whereas the expression levels of myosin heavy chain Ⅱb (MyHCⅡb) in the BF muscle and myostatin (MSTN) in the BF and PM muscles were downregulated in the probiotics group (P<0.05); the expression levels of Myf6, myogenic differentiation factor, and MyoG in the BF muscle and Myf5 and MyoG in the PM muscle were upregulated in the synbiotics group (P<0.05), whereas those of MSTN in the BF muscle were downregulated (P<0.05); the expression levels of MyHCⅡx in the BF muscle were downregulated in the probiotics and synbiotics groups (P<0.05); the expression levels of MyHCⅡx in the PM muscle were upregulated whereas MyHCⅠ in the BF muscle and MyHCⅡa in the PM muscle were downregulated in the antibiotic group (P<0.05) . [Conclusions] Maternal addition with probiotics and synbiotics could alter muscular amino acids composition, regulate the expression of genes related to growth factors, upregulate oxidative muscle fiber gene expression, and downregulate glycolytic muscle fiber gene expression, which were beneficial to improving the meat quality of offspring. [ABSTRACT FROM AUTHOR]

Details

Language :
Chinese
ISSN :
10002030
Volume :
47
Issue :
6
Database :
Academic Search Index
Journal :
Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao
Publication Type :
Academic Journal
Accession number :
181421087
Full Text :
https://doi.org/10.7685/jnau.202310020