Load‐dependence of the activation of myosin filaments in heart muscle.

Contraction of heart muscle requires activation of both the actin and myosin filaments. The mechanism of myosin filament activation is unknown, but the leading candidate hypothesis is direct mechano‐sensing by the filaments. Here, we tested this hypothesis by activating intact trabeculae from rat heart by electrical stimulation under different loads and measuring myosin filament activation by X‐ray diffraction. Unexpectedly, we found that the distinct structural changes in the myosin filament associated with activation had different dependences on the load. In early activation, all the structural changes indicated faster activation at higher load, as expected from the mechano‐sensing hypothesis, but, at later times, the helical order of the myosin motors characteristic of the inactivated state was lost even at very low load. We conclude that mechano‐sensing does operate in heart muscle, but it is supplemented by a previously undescribed mechanism that links myosin filament activation to actin filament activation. Key points: Myosin filament activation controls the strength and speed of contraction in heart muscle.Early activation of the myosin filament is determined by the filament load.At later times, myosin filament activation is controlled by a load independent pathway.This load independent pathway provides new targets and assays for drug development. [ABSTRACT FROM AUTHOR]