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dsRNA-mediated innate immunity of epidermal keratinocytes
- Source :
-
Biochemical & Biophysical Research Communications . Sep2005, Vol. 335 Issue 2, p505-511. 7p. - Publication Year :
- 2005
-
Abstract
- Abstract: MIP-1α, a CC chemokine, recruits monocytes, natural killer cells, lymphocytes, and neutrophils, and plays a critical role in viral infection. Since, the lesional epidermis of herpes zoster expressed MIP-1α, we hypothesized that keratinocytes produce MIP-1α in response to virus-associated dsRNA via TLR3. To investigate this, we examined cultured human keratinocytes for MIP-1α production induced by poly(I:C), a TLR3 ligand. Poly(I:C) treatment induced MIP-1α production, interestingly, poly(I:C)-induced IFN-α and -β production preceded MIP-1α production. A neutralizing antibody for IFN-β significantly inhibited the poly(I:C)-induced MIP-1α production indicating that MIP-1α production is via IFN-β. IFN-α priming enhanced TLR3 expression and MIP-1α production in poly(I:C)-treated keratinocytes. This suggests that IFN-α enhanced the TLR3 expression and reinforced the response of keratinocytes to poly(I:C), which resulted in an increase in MIP-1α production. In conclusion, normal human keratinocytes produce MIP-1α in response to dsRNA via TLR3, and this production is regulated by IFN-α/β. [Copyright &y& Elsevier]
- Subjects :
- *RNA
*KERATINOCYTES
*KILLER cells
*NEUTROPHILS
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 335
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 18195526
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.07.105