Back to Search
Start Over
Simultaneous determination of quercetin, kaempferol and isorhamnetin accumulated human beast cancer cells, by high-performance liquid chromatography
- Source :
-
Journal of Pharmaceutical & Biomedical Analysis . Sep2005, Vol. 39 Issue 1/2, p328-333. 6p. - Publication Year :
- 2005
-
Abstract
- Abstract: Quercetin, kaempferol and isorhamnetin are the most important constituents in ginkgo flavonoids. A simple, rapid and sensitive high-performance liquid chromatography method was developed to simultaneously determine quercetin, kaempferol and isorhamnetin absorped by human breast cancer cells. Cells were treated with ginkgo flavonols and then lysed with Triton-X 100. The flavonols in the samples were measured by RP-HPLC with a C18 column after a simple extraction with a mixture of ether and acetone. The mobile phase contained phosphate buffer (pH 2.0; 10mM) tetrahydrofuran, methanol and isopropanol (65:15:10:20, v/v/v/v). The ultraviolet detector was operated at 380nm. The calibration curve was linear from 0.1 to 1.0μM (r >0.999) for each flavonol. The mean extraction efficiency was about 70%. The recovery of the assay was between 98.9 and 100.6%. The limit of detection was 0.01μM for quercetin and kaempferol and 0.05μM for isorhamnetin. The limit of quantitation was 0.1μM (R.S.D.<10%) for each flavonol. The intra- and inter-day coefficients of variation were less than 10% (R.S.D.). The validated method was applied to quantify quercetin, kaempferol and isorhamnetin in human breast cancer Bcap37 and Bcap37/MDR1 cells. [Copyright &y& Elsevier]
- Subjects :
- *QUERCETIN
*BIOFLAVONOIDS
*FATTY acids
*POLYPHENOLS
*GINKGO
Subjects
Details
- Language :
- English
- ISSN :
- 07317085
- Volume :
- 39
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Pharmaceutical & Biomedical Analysis
- Publication Type :
- Academic Journal
- Accession number :
- 18204963
- Full Text :
- https://doi.org/10.1016/j.jpba.2005.03.016