GREEN SYNTHESIS AND ANALYTICAL TECHNIQUE FOR THE SEPARATION OF SUBSTITUTED CHLOROPHENYL HYDRAZINE ISOMERS BY REVERSE PHASE HPLC METHOD.

4-Chlorophenylhydrazine hydrochloride (4-CPH) is a crucial intermediate in organic synthesis, utilized in various applications including the production of Carprofen and wastewater treatment. This study focuses on the synthesis and impurity analysis of 4-CPH on green approach, with particular emphasis on the detection and quantification of positional isomers 2-Chlorophenylhydrazine hydrochloride (2-CPH), 3-Chlorophenylhydrazine hydrochloride (3- CPH), and 4-Chloroaniline. A high-performance liquid chromatography (HPLC) method was developed using a Waters X-Bridge C18 column and optimized for resolution and sensitivity. The method demonstrated effective separation, with detection limits for 2-CPH, 3-CPH and 4-Chloroaniline at 0.02%, 0.04%, and 0.02% respectively. The validated HPLC method provides reliable analysis for the quality control of 4-CPH, ensuring low impurity levels in the final product. [ABSTRACT FROM AUTHOR]