Standardisation and physicochemical characterisation of trunk bark extracts of Parkia biglobosa used in the manufacture of plant-based medicines.

This study analyzed the physicochemical characteristics, microbial quality, and antioxidant activities of extracts from Parkia biglobosa trunk bark powders collected in four sites (Gonse, Yako, Bobo, and Nobere) in Burkina Faso. The physicochemical characteristics of the extracts and the microbial quality were studied according to European Pharmacopoeia methods. The antioxidant activity of the extracts was also evaluated. The extraction yields varied from 13.82 for the Nobere site to 17.38 for the Gonse site. The study of phytochemical parameters shows that the extracts from the 4 sites had uniform macroscopic characteristics and pH, and they all had a residual moisture content of less than 10%. As for the microbial analysis, the extracts from the trunk bark of the 4 sites all comply with the specifications of the European Pharmacopoeia. Tannins, flavonoids, coumarins, saponins, triterpenes, and sterols were highlighted in all the extracts. The Bobo and Yako sites statistically had the highest total phenolic contents (558.06±19.69 and 532.40±23.78 EAT/g) and tannins (415.58±15.17 and 408.38±25.54 EAT/g). On the other hand, the 4 extracts had the same contents for flavonoids. Concerning the antioxidant activity, all the extracts from the 4 sites had IC50s lower than 4 µg/ml by the ABTS method, and the extracts from the Bobo, Gonse, and Yako sites had IC50 of 6 µg/ml lower than that of the Nobere site. This study made it possible to choose, through the characterization, quantification, and activity of extracts from harvest sites suitable for the pharmaceutical production of phytomedicines. [ABSTRACT FROM AUTHOR]