十一个 DNA 甲基化位点对 野生与圈养环颈雉鉴别能力的评价.

The overexploitation and illegal hunting of wildlife resources have become the important threats to biodiversity. The incessant practice of blending unlawfully acquired wild animals with legally farmed ones to conceal illicit activities necessitates our ability to discern between the two sources through scientific identification. Skeletal muscle serves as one of the primary targets for illegal hunting and trade products; however, an effective means of identifying their source remains elusive. In this study, we selected the common pheasant (Phasianus colchicus), a novel poultry species classified in the National Catalogue of Livestock and Poultry Genetic Resources as well as being considered as an exemplar case. We employed methylation-sensitive high resolution genetic melting（MS-HRM) assay to test 26 potential methylation level between wild and captive common pheasants at specific sites. We identified 11 loci that could be consistently detected. Statistical analysis revealed that seven sites including LOC116232884, NFATC2, FGF9-3, FGF9-2, MAPK8, DUSP10 and MYCN exhibited significant or highly significant differences in methylation levels between the two groups (P = 0. 000 - 0. 004) . The overall accuracy rate for discriminating each locus ranged from 62. 22% to 82. 78%. Furthermore, the cumulative discrimination power reached 99. 999 93%. The findings not only offer valuable locus resources and detection methods for distinguishing between wild and captive common pheasants but also serve as direct reference for identifying other similar species. [ABSTRACT FROM AUTHOR]