Back to Search
Start Over
Immunoassay using the probe-labeled Au/Ag core-shell nanoparticles based on surface-enhanced Raman scattering
- Source :
-
Colloids & Surfaces A: Physicochemical & Engineering Aspects . May2005, Vol. 257-258, p171-175. 5p. - Publication Year :
- 2005
-
Abstract
- Abstract: Au/Ag core-shell nanoparticles labeled by monoclonal antibody and surface-enhanced Raman scattering (SERS) probes were used for immunoassay analysis. Mouse polyclonal antibodies against hepatitis B surface antigen (mouse Anti-HBsAg PAb) were covalently immobilized on the silicon chips modified by a self-assembled monolayer of (3-amino-propyl) trimethoxysilane via glutaraldehyde (GA) activation. The Au/Ag core-shell nanoparticles were labeled by the mouse monoclonal antibody against hepatitis B surface antigen (mouse Anti-HBsAg MAb) and the probe molecule, p-mercaptobenzoic acid (MBA). Immunoreactions between the labeled Au/Ag core-shell nanoparticles and the corresponding antigens captured by the Anti-HBsAg PAb on the silicon chips were investigated by SERS. [Copyright &y& Elsevier]
- Subjects :
- *IMMUNOASSAY
*RAMAN effect
*LIGHT scattering
*IMMUNOGLOBULINS
Subjects
Details
- Language :
- English
- ISSN :
- 09277757
- Volume :
- 257-258
- Database :
- Academic Search Index
- Journal :
- Colloids & Surfaces A: Physicochemical & Engineering Aspects
- Publication Type :
- Academic Journal
- Accession number :
- 18377276
- Full Text :
- https://doi.org/10.1016/j.colsurfa.2004.10.096