14-3-3 regulation of cell spreading and migration requires a functional amphipathic groove.

The 14-3-3 proteins associate with many cellular proteins that participate in the regulation of various cellular events including apoptosis, the cell cycle, spreading, and migration. We have previously described that 14-3-3 binds the 1-integrin and overexpression of 14-3-3 promoted increased cell spreading and migration (Han et al. [2001] Oncogene 20: 346357). In this study, we find that mutation of Ser 60 of 14-3-3, outside of the amphipathic groove which is involved in 14-3-3 protein interactions with other ligands, abolished its interaction with integrin. Surprisingly, this mutant retained its ability to promote cell spreading, suggesting that 14-3-3 interaction with the 1-integrin is not required for its regulation of cell adhesion. We next showed that mutations of several critical residues in the amphipathic groove did not affect 14-3-3 interaction with the 1-integrin. As expected, these mutants disrupted their association with the phosphoserine dependent ligands Raf and Cas. Analysis of the groove mutant LF (mutation of Arg129Tyr130 to Leu and Phe) indicated that, unlike wild type 14-3-3, it could not stimulate cell spreading or migration, suggesting that a functional amphipathic groove is required for 14-3-3 regulation of cell adhesion and migration. Consistent with this, cells expressing the LF mutant exhibited a delay in F-actin organization compared to cells expressing wild type or the S60A mutant (Ser 60 to Ala mutation) upon cell adhesion to fibronectin (FN). Taken together, these studies identified a novel binding site on 14-3-3 for integrin 1 and showed that a functional amphipathic groove, rather than its interaction with integrin 1, is required for 14-3-3 regulation of cell spreading and migration. 2005 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]