Back to Search
Start Over
Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex.
- Source :
-
Acta Crystallographica: Section F (Wiley-Blackwell) . Jan2005, Vol. 61 Issue 1, p59-61. 3p. - Publication Year :
- 2005
-
Abstract
- In living systems, the intramolecular cyclization of N-terminal glutamine residues is accomplished by glutaminyl cyclase enzymes (EC 2.3.2.5). While in mammals these enzymes are involved in the synthesis of hormonal and neurotransmitter peptides, the physiological role played by the corresponding plant enzymes still remains to be unravelled. Papaya glutaminyl cyclase (PQC), a 33 kDa enzyme found in the latex of the tropical tree Carica papaya, displays an exceptional resistance to chemical and thermal denaturation as well as to proteolysis. In order to elucidate its enzymatic mechanism and to gain insights into the structural determinants underlying its remarkable stability, PQC was isolated from papaya latex, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 62.82, b = 81.23, c = 108.17 Å and two molecules per asymmetric unit. Diffraction data have been collected at ESRF beamline BM14 and processed to a resolution of 1.7 Å. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CRYSTALLIZATION
*X-ray diffraction
*TRANSGLUTAMINASES
*TRANSFERASES
*CARICA
*PAPAYA
Subjects
Details
- Language :
- English
- ISSN :
- 17443091
- Volume :
- 61
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Acta Crystallographica: Section F (Wiley-Blackwell)
- Publication Type :
- Academic Journal
- Accession number :
- 18502639
- Full Text :
- https://doi.org/10.1107/S1744309104025904