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Determinants of the nucleolar targeting of protein phosphatase-1
- Source :
-
FEBS Letters . Oct2005, Vol. 579 Issue 25, p5626-5630. 5p. - Publication Year :
- 2005
-
Abstract
- Abstract: The ubiquitously expressed protein Ser/Thr phosphatase-1 isoforms PP1α, PP1β and PP1γ1 are dynamically targeted to distinct, but overlapping cellular compartments by associated proteins. Within the nucleus of HeLa cells, EGFP-tagged PP1γ1 and PP1β were predominantly targeted to the nucleoli, while PP1α showed a more diffuse distribution. Using PP1 chimaeras and point mutants we show here that a single N-terminal residue, i.e., Gln20 for PP1α, Arg19 for PP1β and Arg20 for PP1γ1 accounts for their distinct subnuclear distribution. Our data also suggest that the N-terminus of PP1β and PP1γ1 harbours an interaction site for one or more nucleolar interactors. [Copyright &y& Elsevier]
- Subjects :
- *CELL nuclei
*CLONE cells
*CELL culture
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 579
- Issue :
- 25
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 18781993
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.09.033