Isolation and characterization of 27 polymorphic microsatellite loci for the common snook, Centropomus undecimalis.

Here we describe 32 di-, tri- and tetranucleotide microsatellite loci isolated by PIMA, a polymerase chain reaction (PCR)-based procedure, for the common snook ( Centropomus undecimalis). Five loci were monomorphic, and the remaining loci averaged 6.7 alleles per locus in a sample of 45 common snook. For polymorphic loci, expected heterozygosities ranged from 0.02 to 0.91 (mean = 0.538). Significant departures from Hardy–Weinberg equilibrium expectations occurred in two loci. Exact tests for genotype disequilibrium gave evidence for linkage at one pair of loci. Many cross-species primer assays yielded PCR fragments of the expected size for 11 species of Centropomus and two species of the confamilial genus Lates. [ABSTRACT FROM AUTHOR]