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Simultaneous determination of 6β-hydroxycortisol and cortisol in human urine and plasma by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Nov2005, Vol. 826 Issue 1/2, p238-243. 6p. - Publication Year :
- 2005
-
Abstract
- Abstract: This study describes an high-performance liquid chromatographic (HPLC)–UV method for the simultaneous determination of 6β-hydroxycortisol (6β-OHF) and cortisol (F) in human urine and plasma. The within-day relative standard deviation of the three concentrations for both analytes was less than 6.2%. Accuracy determined at three concentrations ranged between 95 and 107%. The extraction recoveries were 64.1±4.3 and 88.1±2.4% at three concentrations for 6β-OHF and F in urine, respectively. The extraction recoveries were 88.7±1.4% at three concentrations for F in plasma. This is the first HPLC method that can simultaneously determine 6β-OHF and F in human urine and plasma and is suitable for routine assessment of the CYP3A activity expressed as 6β-hydroxylation clearance. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 826
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 18907980
- Full Text :
- https://doi.org/10.1016/j.jchromb.2005.09.007