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Acyl chain length affects ceramide action on sterol/sphingomyelin-rich domains
- Source :
-
BBA: Biomembranes . Dec2005, Vol. 1718 Issue 1/2, p61-66. 6p. - Publication Year :
- 2005
-
Abstract
- Abstract: The effects of ceramides with varying saturated N-linked acyl chains (C2–C14) on cholesterol displacement from sphingomyelin-rich domains and on the stability of ordered domains were studied. The bilayers examined were made from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), d-erythro-N-palmitoyl-sphingomyelin (PSM), d-erythro-N-acyl-sphingosine, and cholesterol (60:15:15:10 mol%, respectively). Cholestatrienol (CTL) or d-erythro-N-trans-parinoyl-sphingomyelin (tParSM) were used as reporter molecules (at 1 mol%) for the ordered domains, and 1-palmitoyl-2-stearoyl-(7-doxyl)-sn-glycero-3-phosphocholine (7SLPC) as a fluorescence quencher (30 mol%, replacing POPC) in the liquid-disordered phase. The results indicate that the ceramide had to have an N-linked acyl chain with at least 8 methylene units in order for it to displace cholesterol from the sphingomyelin-rich domains at the concentration used. The melting of the sphingomyelin-rich domain shifted to higher temperatures (compared to the ceramide-free control) with C2, C12 and longer chain ceramides, whereas C4–C10 ceramides led to domain melting at lower temperatures than control. This study shows that short-chain ceramides do not have the same effects on sterol- and sphingomyelin-rich domains as naturally occurring longer-chain ceramides have. [Copyright &y& Elsevier]
- Subjects :
- *STEROLS
*CERAMIDES
*CHOLESTEROL
*LIPIDS
Subjects
Details
- Language :
- English
- ISSN :
- 00052736
- Volume :
- 1718
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- BBA: Biomembranes
- Publication Type :
- Academic Journal
- Accession number :
- 19128542
- Full Text :
- https://doi.org/10.1016/j.bbamem.2005.10.009