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An NMR Approach Applicable to Biomolecular Structure Characterization.
- Source :
-
Analytical Chemistry . 12/1/2005, Vol. 77 Issue 23, p7534-7538. 5p. - Publication Year :
- 2005
-
Abstract
- A generalized 2D correlation NMR (GEN2D-NMR) scheme capable of substantially reducing the experimental time for two-dimensional correlation NMR experiments is described. The experimental time used in GEN2D-NMR is shortened to less than one-tenth of that required in traditional double Fourier transform 2D-NMR (FT2D- NMIR) for a 13C-13C spin diffusion experiment for Nephila edulis spider threads. Experimentally, one needs to acquire many fewer points in GEN2D-NMR than that in FT2D-NMR in the evolution time domain. By keeping other acquisition pulse sequence and parameters the same in both methods, the GEN2D-NMR technique can produce a 2D-NMR spectrum using fewer points along the evolution time domain equivalent to that produced by the FT2D-NMR technique using more points. GEN2D- NMR would provide a significant advantage for those molecules that are difficult to highly isotropically label, such as proteins, polypeptides, and polymers, or those which become unstable in a prolonged measurement time. [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEINS
*PEPTIDES
*BIOMOLECULES
*ORGANIC compounds
*POLYMERS
*MACROMOLECULES
Subjects
Details
- Language :
- English
- ISSN :
- 00032700
- Volume :
- 77
- Issue :
- 23
- Database :
- Academic Search Index
- Journal :
- Analytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19327489
- Full Text :
- https://doi.org/10.1021/ac051061o