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NF-κB activation is required for cisplatin-induced apoptosis in head and neck squamous carcinoma cells
- Source :
-
FEBS Letters . Jan2006, Vol. 580 Issue 1, p311-318. 8p. - Publication Year :
- 2006
-
Abstract
- Abstract: This study demonstrates a requirement for NF-κB activation in cis-diamminedichloroplatinum (cisplatin)-induced apoptosis in human head and neck squamous cell carcinoma (HNSCC) cell lines. This conclusion was supported by the following observations: cisplatin induced IκBα degradation and NF-κB-dependent transcriptional activation prior to cell death; pyrrolidine dithiocarbamate (PDTC), a chemical inhibitor of NF-κB activation, prevented apoptosis; lactacystin, an inhibitor of IκBα degradation, also prevented apoptosis; and finally, the expression of a super-repressor mutant IκBα blocked apoptosis. The expression of tumor necrosis factor α (TNFα) was promoted by cisplatin treatment and was suppressed by PDTC treatment. In addition, a neutralizing antibody against TNFα protected cells from cisplatin-induced apoptosis. These findings suggest that NF-κB activation is required for cisplatin-induced apoptosis and TNFα may play an important role in NF-κB-mediated apoptosis in cisplatin-treated HNSCC cell lines. [Copyright &y& Elsevier]
- Subjects :
- *CISPLATIN
*ALKYLATING agents
*SQUAMOUS cell carcinoma
*APOPTOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Volume :
- 580
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- FEBS Letters
- Publication Type :
- Academic Journal
- Accession number :
- 19339904
- Full Text :
- https://doi.org/10.1016/j.febslet.2005.12.012