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Analysis of HIV-1 subtype B third variable region peptide motifs for induction of neutralizing antibodies against HIV-1 primary isolates

Authors :
Haynes, Barton F.
Ma, Benjiang
Montefiori, David C.
Wrin, Terri
Petropoulos, Christos J.
Sutherland, Laura L.
Scearce, Richard M.
Denton, Cathrine
Xia, Shi-Mao
Korber, Bette T.
Liao, Hua-Xin
Source :
Virology. Feb2006, Vol. 345 Issue 1, p44-55. 12p.
Publication Year :
2006

Abstract

Abstract: The HIV-1 gp120 V3 loop is a potent inducer of neutralizing antibodies for T cell line adapted-HIV-1, but less so for primary isolates. We hypothesized that peptides representative of the diversity of natural HIV-1 V3 loop variants might capture elements of conserved higher order structures and so stimulate broadly reactive neutralizing antibodies. We designed a panel of 29 subtype B V3 sequences postulated to reflect the range of V3 diversity. These peptides were used to immunize guinea pigs. The most effective peptide (62.19) clustered around the subtype B consensus sequence and induced antibodies that reproducibly neutralized 31% of the subtype B HIV-1 primary isolates evaluated, but exhibited limited cross-neutralization of non-subtype B HIV-1 strains. Taken together, these data demonstrated that the limited neutralization profile of antibodies induced by optimal subtype B V3 motifs likely represents the maximum breadth of neutralization of subtype B HIV-1 primary isolates attainable by anti-V3 peptide antibodies. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00426822
Volume :
345
Issue :
1
Database :
Academic Search Index
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
19463582
Full Text :
https://doi.org/10.1016/j.virol.2005.08.042