Back to Search Start Over

Selective elimination of HIV-1-infected cells by Env-directed, HIV-1-based virus-like particles

Authors :
Peretti, Silvia
Schiavoni, Ilaria
Pugliese, Katherina
Federico, Maurizio
Source :
Virology. Feb2006, Vol. 345 Issue 1, p115-126. 12p.
Publication Year :
2006

Abstract

Abstract: We recently showed that both replicating and resting cells cultivated with ganciclovir (GCV) were killed when challenged with vesicular stomatitis virus G glycoprotein pseudotyped HIV-1-based virus-like particles (VLPs) carrying the Nef7 (i.e., an HIV-1 Nef mutant incorporating in virions at high levels)/herpes simplex virus-1 thymidine kinase (HSV-TK) fusion product. On this basis, a novel anti-HIV therapeutic approach based on Nef7/TK VLPs expressing X4 or R5 HIV cell receptor complexes has been attempted. We here report that (CD4-CXCR4) and (CD4-CCR5) Nef7-based VLPs efficiently enter cells infected by X4- or R5-tropic HIV-1 strains, respectively. Importantly, the delivery of the VLP-associated Nef7/TK led to cell death upon GCV treatment. Of interest, VLPs were effective also against non-replicating, HIV-1-infected primary human monocyte-derived macrophages. HIV-targeted VLPs represent a promising candidate for the treatment of persistently HIV-1-infected cells that are part of virus reservoirs resistant to HAART therapies. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
00426822
Volume :
345
Issue :
1
Database :
Academic Search Index
Journal :
Virology
Publication Type :
Academic Journal
Accession number :
19463589
Full Text :
https://doi.org/10.1016/j.virol.2005.09.054