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U50488 inhibits outwardly rectifying potassium channel in PC12 cells via pertussis toxin-sensitive G-protein
- Source :
-
Biochemical & Biophysical Research Communications . Feb2006, Vol. 340 Issue 4, p1184-1191. 8p. - Publication Year :
- 2006
-
Abstract
- Abstract: This study was undertaken to determine the effect of U50488, a κ-opioid receptor agonist, on outwardly rectifying potassium channel (Ik) in undifferentiated PC12 cells. Using whole-cell and on-cell patch–clamp techniques, we found that U50488 decreased Ik amplitude in a time-dependent manner and Ik activation was delayed. Single-channel kinetic analysis provided a two-stage model for us to illuminate the blockage effect induced by U50488. To identify whether U50488 mediates the effect through opioid receptor and G-protein, several specific blockers and activators were used. Not only naloxone but also PTX and GDPβS abolished U50488-induced suppression; however, such effect was not observed when cAMP or other adenylyl cyclase activators were used. It is postulated that κ-opioid receptor and Gi/o protein, but not cAMP, are involved in U50488-induced suppression of Ik. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 340
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 19463631
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.12.134