Association of the C-344T polymorphism of CYP11B2 gene with essential hypertension in Hani and Yi minorities of China

Abstract: Background: Aldosterone synthase (CYP11B2) is a key enzyme in the biosynthesis of aldosterone. Recently, a C-344T polymorphism in the promoter region of the CYP11B2 gene has been reported to be in association with high blood pressure. We investigated the association between this polymorphism and essential hypertension in Hani (n =305 individuals) and Yi (n =233 individuals) minorities of China. Methods: CYP11B2 genotyping with polymerase chain reaction–restriction fragment length polymorphism was performed in 267 normotensive subjects and 271 essential hypertensive subjects. At the same time, the T(-344)C polymorphism detection in 33 subjects was also performed by sequencing. Result: The frequency of CYP11B2 C-344T genotype in normotensive controls and essential hypertensive cohort in Hani population were TT: 0.729 vs. 0.610; CT+CC: 0.271 vs. 0.390, respectively. The frequency of CYP11B2 C-344T genotype in normotensive controls and essential hypertensive cohort in Yi population were TT: 0.612 vs. 0.475; CT+CC: 0.388 vs. 0.525, respectively. The frequency of CC+CT genotype in the essential hypertensive group was significantly higher than that in the normotensive controls in both Hani and Yi populations (P <0.05). Conclusion: The -344C allele of the CYP11B2 may play a role in genetic predisposition to developing essential hypertension in Hani and Yi minorities of China. [Copyright &y& Elsevier]