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Single amino-acid InDel variants generated by alternative tandem splice-donor and -acceptor selection
- Source :
-
Biochemical & Biophysical Research Communications . Mar2006, Vol. 342 Issue 1, p197-205. 9p. - Publication Year :
- 2006
-
Abstract
- Abstract: We have investigated putative single amino-acid InDel variants with human ESTs. Examination of the formation process for single amino-acid InDel variants indicates a possible splicing mechanism in addition to the genomic insertion/deletion events as would be expected. The wobble-splicing transcripts were often generated around the intron–exon boundaries by selecting an alternative neighboring splice signal sequence, in particular the tandem agNAG or GTNgt sequence at the splice-acceptor or -donor site, thus creating single amino-acid InDel isoforms. Another category of variants was identified with one altered amino-acid plus one amino-acid InDel, under divergent coding-frame usage. We demonstrate that such minute distance of splice site choice generates an even greater level of transcriptome diversity, and suggest that non-functional synonymous or intronic SNPs could be converted to functionally significant InDel alterations through this process. This subtle alteration in mRNA and protein-coding sequence may elicit a great impact upon human genome and proteome diversity. [Copyright &y& Elsevier]
- Subjects :
- *AMINO acids
*GENOMES
*MESSENGER RNA
*HUMAN chromosomes
*GENETIC research
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 342
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 19766132
- Full Text :
- https://doi.org/10.1016/j.bbrc.2006.01.101