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The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway.
- Source :
-
Science . 2/10/2006, Vol. 311 Issue 5762, p822-826. 5p. 3 Diagrams, 3 Graphs, 3 Maps. - Publication Year :
- 2006
-
Abstract
- Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active — it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00368075
- Volume :
- 311
- Issue :
- 5762
- Database :
- Academic Search Index
- Journal :
- Science
- Publication Type :
- Academic Journal
- Accession number :
- 19837868
- Full Text :
- https://doi.org/10.1126/science.1120941