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A hyper-dynamic equilibrium between promoter-bound and nucleoplasmic dimers controls NF-κB-dependent gene activity.
- Source :
-
EMBO Journal . 2/22/2006, Vol. 25 Issue 4, p798-810. 13p. 1 Diagram, 6 Graphs. - Publication Year :
- 2006
-
Abstract
- Because of its very high affinity for DNA, NF-κB is believed to make long-lasting contacts with cognate sites and to be essential for the nucleation of very stable enhanceosomes. However, the kinetic properties of NF-κB interaction with cognate sites in vivo are unknown. Here, we show that in living cells NF-κB is immobilized onto high-affinity binding sites only transiently, and that complete NF-κB turnover on active chromatin occurs in less than 30 s. Therefore, promoter-bound NF-κB is in dynamic equilibrium with nucleoplasmic dimers; promoter occupancy and transcriptional activity oscillate synchronously with nucleoplasmic NF-κB and independently of promoter occupancy by other sequence-specific transcription factors. These data indicate that changes in the nuclear concentration of NF-κB directly impact on promoter function and that promoters sample nucleoplasmic levels of NF-κB over a timescale of seconds, thus rapidly re-tuning their activity. We propose a revision of the enhanceosome concept in this dynamic framework. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENES
*DNA
*NUCLEATION
*DIMERS
*TRANSCRIPTION factors
*CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 02614189
- Volume :
- 25
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- EMBO Journal
- Publication Type :
- Academic Journal
- Accession number :
- 19864245
- Full Text :
- https://doi.org/10.1038/sj.emboj.7600977