Detection of radiation-induced markers from parts of irradiated kiwifruits

Abstract: Parts of irradiated kiwifruits were investigated for radiation-induced markers. The DNA comet assay on seeds showed that non-irradiated cells appeared as intact nuclei without tails, while irradiated cells displayed comets with long tails. Tail length of seeds increased significantly up to 1kGy (p <0.05), showing a positive correlation (R 2 =0.9084) between the irradiation dose and tail length. The triplet signals from cellulose radicals observed in electron spin resonance (ESR) analysis on the core or flesh of kiwi enabled irradiated samples to be distinguished from the non-irradiated samples. The ESR signals increased in correlation with the irradiation doses with R 2 =0.9071 in the core and R 2 =0.9730 in flesh. Both methods were found suitable for the detection of irradiated kiwi during the six-week shelf-life period. [Copyright &y& Elsevier]