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A NEW MONOCLONAL ANTIBODY RADIOPHARMACEUTICAL FOR RADIOIMMUNOSCINTIGRAPHY OF BREAST CANCER: DIRECT LABELING OF ANTIBODY AND ITS QUALITY CONTROL.
- Source :
-
DARU: Journal of Pharmaceutical Sciences . 2006, Vol. 14 Issue 1, p51-56. 6p. - Publication Year :
- 2006
-
Abstract
- Radioimmunoscintigraphy (RIS) has found widespread clinical application in tumor diagnosis. The antibody (Ab) PR81 is a new murine anti-MUC1 monoclonal antibody (MAb) against human breast carcinoma. In this study a very simple, rapid and efficient method for labeling of this MAb with 99mTc, particularly suitable for development of a 'kit'is described. The reduction of Ab was performed with 2- mercaptoethanol (2-ME) at a molar ratio of 2000:1 (2-ME:MAb) and the reduced Ab was labeled with 99mTc via methylene diphosphonate (MDP) as a transchelator. The labeling efficiency which was determined by instant thin layer chromatography (ITLC) was 94.2%±2.3. Radiocolloides measured by cellulose nitrate electrophoresis were 2.5%±1.7. In vitro stability of the labeled product in human serum which was measured by gel filtration chromatography (FPLC) was 70%±5.7 over 24 hr. The integrity of labeled MAb was checked by means of SDS-PAGE and no significant fragmentation was observed. The results of the cell-binding studies showed that both labeled and unlabeled PR81 were able to compete for binding to MCF 7 cells. Biodistribution studies were performed in normal BALB/c mice at 4 and 24 hrs post-injection and no important accumulation was observed in vital organs. These results show that the new radiopharmaceutical may be considered as a promising candidate for imaging of breast cancer. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15608115
- Volume :
- 14
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- DARU: Journal of Pharmaceutical Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 20220499