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Hybrid lethality in interspecific hybrids between Nicotiana tabacum and N. suaveolens: evidence that the Q chromosome causes hybrid lethality based on Q-chromosome-specific DNA markers.
- Source :
-
Theoretical & Applied Genetics . Apr2006, Vol. 112 Issue 6, p1172-1178. 7p. 1 Color Photograph, 1 Diagram, 3 Charts, 1 Graph. - Publication Year :
- 2006
-
Abstract
- Hybrid seedlings from the cross Nicotiana tabacum × N. suaveolens express lethality at 28°C. We carried out a cross between monosomic lines of N. tabacum lacking the Q chromosome and N. suaveolens by test-tube pollination and ovule culture at 28°C. To suppress hybrid lethality, hybrid seedlings obtained were transferred to 36°C immediately after germination and cultured. We determined whether Q-chromosome-specific DNA markers were detected among hybrid seedlings. When hybrid seedlings cultured at 36°C were transferred to 28°C, hybrid seedlings in which Q-chromosome-specific DNA markers were detected expressed hybrid lethality, while hybrid seedlings in which Q-chromosome-specific DNA markers were not detected did not express hybrid lethality. From these results, we concluded that the presence of the Q chromosome of N. tabacum is related to hybrid lethality observed in crosses between N. tabacum and N. suaveolens. This is the first report that clearly demonstrates the relationship between a certain chromosome and hybrid lethality in the genus Nicotiana using chromosome-specific DNA markers. Additionally, we confirmed that the Q chromosome belongs to the S subgenome because Q-chromosome-specific DNA markers were detected only in N. sylvestris. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TOBACCO
*CHROMOSOMES
*GENETIC markers
*POLLINATION
*GERMINATION
Subjects
Details
- Language :
- English
- ISSN :
- 00405752
- Volume :
- 112
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Theoretical & Applied Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 20308814
- Full Text :
- https://doi.org/10.1007/s00122-006-0219-0