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Somatic Antigens of Pseudomonas aeruginosa The Structure of the O-Specific Polysaccharide Chains of Ps.aeruginosa O:2 (Lanyi) Lipopolysaccharides.
- Source :
-
European Journal of Biochemistry . 6/15/82, Vol. 125 Issue 1, p221-227. 7p. 2 Charts, 2 Graphs. - Publication Year :
- 1982
-
Abstract
- Structural and immunochemical studies have been performed on the O-specific antigens of two serologically related types of Pseudomonas aeruginosa O:2a,b and O:2a,c (Lanyi's classification). The O-specific polysaccharide chains of the two serotypes were shown to be acidic polysaccharides composed of repeating trisaccharide units consisting of L-rhamnose, N-acetyl-D-quinovosamine and N-acetyl-L-galactosaminuronic acid residues. Based on 1H and 13C nuclear magnetic resonance spectroscopy, data of methylation analysis and selective solvolysis with hydrogen fluoride, the repeating unit of the O:2a,c O-specific polysaccharide was assigned the following structure: →4)LGalNAcA(α1 → 3)DQuiNAc(α1 » 3)LRha(α1, where GalNAcA = N-acetylgalactosaminuronic acid, QuiNAc = N-acetylquinovosamine and Rha = rhamnose. The O:2a,b O-specific polysaccharide had the same structure of the trisaccharide repeating unit but was distinguished by the presence of O-acetyl groups on 70–80% of the rhamnose residues in position 2. The O-acetyl groups were located both by methylation with methyltrifluoromethane sulphonate and by comparison of the 13C nuclear magnetic resonance spectra of the native and O-deacetylated polysaccharides. Serological studies revealed the determinant role of the O-acetyl groups in the O-antigen O:2a,b and suggested the O-factor 2b to be related to the 2-O-acetyl-L-rhamnose residue, whereas the O-factor 2c was probably determined by the nonacetylated rhamnose residue. The dominant moiety of the determinant 2a common to the two antigens was obviously presented by the N-acetyl-L-galactosaminuronic acid residue. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 125
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 20393088
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1982.tb06672.x