Comparison of paternity indices based on typing of 15 STRs, 7 VNTRs and 52 SNPs in 50 Danish mother–child–father trios

Abstract: Fifty Danish paternity cases from the year 2004 were selected based on the results obtained with the AmpFlSTR® Identifiler® PCR amplification kit. In all cases, the calculated paternity index (PI) was higher than 10,000, and there was not observed any genetic inconsistency between mother and child, or between father and child. DNA from the selected trios was used to type 7 VNTRs (D2S44, D5S43, D5S110, D7S21, D7S22, D12S11 and D16S309) with the RFLP technique and 52 SNPs using a PCR multiplex with 52 PCR primer pairs and two SBE multiplexes with 23 and 29 SBE primers, respectively. PIs were calculated based on each set of loci (STRs, VNTRs and SNPs). On average, the typical PI obtained from analyses of 15 STRs was 4 times higher than the typical PI obtained from analyses of 7 VNTRs, and 17 times higher than the typical PI obtained from analyses of 52 SNPs. However, in only 3 trios analyzed for SNPs and 1 trio analyzed for VNTRs, PI<10,000. No genetic inconsistency was detected between mother and child or between father and child in the SNP-analyses. In contrast, one father–child and one mother–child genetic inconsistency were observed in the VNTR-analyses. [Copyright &y& Elsevier]