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Simultaneous detection of HCV and HIV-1 by SYBR Green real time multiplex RT-PCR technique in plasma samples
- Source :
-
Molecular & Cellular Probes . Jun2006, Vol. 20 Issue 3/4, p223-229. 7p. - Publication Year :
- 2006
-
Abstract
- Abstract: This paper describes the development of a SYBR Green-based multiplex real time RT-PCR for the simultaneous detection of HCV and HIV-1 genomes in plasma samples. Viral genomes were identified in the same sample by their distinctive melting temperature (Tm) which are 81.6 and 86.5°C for HIV-1 gag 142bp amplicon and HCV 5′-NCR region 226bp amplicon, respectively. Analysis of known scalar concentrations of reference plasma indicated that the multiplex procedure detects at least 500copies/ml of both HIV-1 and HCV. In addition, we also assayed HIV-1 and HCV viral load in 30 co-infected patients and in 15 blood donors, confirming the sensitivity and specificity of the assay. This method may represent a useful alternative method for the detection of HIV-1/HCV co-infection, reliable for a rapid and relatively inexpensive screening of blood donors. [Copyright &y& Elsevier]
- Subjects :
- *HIV
*GENOMES
*BLOOD plasma
*VIRAL genomes
Subjects
Details
- Language :
- English
- ISSN :
- 08908508
- Volume :
- 20
- Issue :
- 3/4
- Database :
- Academic Search Index
- Journal :
- Molecular & Cellular Probes
- Publication Type :
- Academic Journal
- Accession number :
- 20768270
- Full Text :
- https://doi.org/10.1016/j.mcp.2005.12.005